A free consultation is provided at the start of the project to discuss experimental design, scope of analysis, and scientific objectives.
For the majority of GAC projects, project costs are based on time and effort for the team performing the analysis. Some examples of project costs are provided below.
RNA Seq: typical pipeline for an RNA Seq experiment with 9 sample, 3 conditions and 3 replicates per group will involve quality control checks, mapping, transcript quantitation and differential expression analysis. If no problems are encountered at any of these steps, the approximate cost may be $2000. sequencing. Sequencing core (GRC) costs are separate from bioinformatics analysis (GAC) prices.
Further analysis for detecting alternate splicing, fusion proteins or pathway analysis, preparation of figures for manuscript will require additional effort at additional cost.
ATAC Seq: QC, mapping, peak calling, visualiziation, motif analysis may require several months of effort and estimated to cost approximately $3000.
Single cell RNA Seq: Cell Ranger, Seurat and marker analysis may require several months of effort and estimated to cost approximately $3000.
Multi-platform integrative analysis: such as ATAC Seq, ChiP Seq and RNA Seq are effort intensive and cost will depend on scope of analysis. A project with 6 samples may require 3-6 months of effort and may cost $5000 - $6000.
Each analysis is a custom analysis; effort involved is determined by the complexity of experimental design, number of samples, number of groups for comparison, scientific objectives, quality of samples, quality of samples, sequencing metrics, platform and application. Investigators are advised to budget for bioinformatics analysis during the experimental planning stage and prior to submission of samples for sequencing.
Please contact Uma Chandran (chandran@pitt.edu) before initiating the project, for detailed discussion about analysis pricing.
We are a Health Sciences Core Facility and bill monthly through their mechanism. Accounts can be created at www.hscrf.pitt.edu or just click the “Login” tab. You may already have an account if you have used another core facility.